论文标题 / A glycerophospholipid-specific pocket in the RVFV class II fusion protein drives target membrane insertion
期刊 / Science
发表时间 / 2017-11-03
数字识别码 / 10.1126/science.aal2712
The Rift Valley fever virus (RVFV) is transmitted by infected mosquitoes, causing severe disease in humans and livestock across Africa. We determined the x-ray structure of the RVFV class II fusion protein Gc in its postfusion form and in complex with a glycerophospholipid (GPL) bound in a conserved cavity next to the fusion loop. Site-directed mutagenesis and molecular dynamics simulations further revealed a built-in motif allowing en bloc insertion of the fusion loop into membranes, making few nonpolar side-chain interactions with the aliphatic moiety and multiple polar interactions with lipid head groups upon membrane restructuring. The GPL head-group recognition pocket is conserved in the fusion proteins of other arthropod-borne viruses, such as Zika and chikungunya viruses, which have recently caused major epidemics worldwide.
（导读 陈月欣） 在非洲，RVF 病毒通过蚊子叮咬在人类和畜类间传播疾病。本研究通过分析 RVF 病毒 II 类融合蛋白 Gc 融合后的结构，发现蛋白内置的模块将数个融合环同时插入细胞膜改变膜结构并与甘油磷脂（GPL）结合。这样的融合蛋白结构在虫媒病毒中高度保守。