论文标题 / An algal photoenzyme converts fatty acids to hydrocarbons
期刊 / Science
发表时间 / 2017-09-01
数字识别码 / 10.1126/science.aan6349
Although many organisms capture or respond to sunlight, few enzymes are known to be driven by light. Among these are DNA photolyases and the photosynthetic reaction centers. Here, we show that the microalga Chlorella variabilis NC64A harbors a photoenzyme that acts in lipid metabolism. This enzyme belongs to an algae-specific clade of the glucose-methanol-choline oxidoreductase family and catalyzes the decarboxylation of free fatty acids to n-alkanes or -alkenes in response to blue light. Crystal structure of the protein reveals a fatty acid–binding site in a hydrophobic tunnel leading to the light-capturing flavin adenine dinucleotide (FAD) cofactor. The decarboxylation is initiated through electron abstraction from the fatty acid by the photoexcited FAD with a quantum yield >80%. This photoenzyme, which we name fatty acid photodecarboxylase, may be useful in light-driven, bio-based production of hydrocarbons.
（导读 阿金）目前已知的光解酶数量很少，其中包括 DNA 光解酶和光合反应中心。本研究报道了一种来自小球藻（Chlorella variabilis ）NC64A 的光解酶，它属于葡萄糖-甲醇-胆碱氧化还原酶家族的特殊藻类分支，通过蓝光照射在脂质代谢中可催化游离脂肪酸脱羧为正烷烃或烯烃 。这种光解酶可用于由光驱动的生物碳氢化合物的生产。